The two white regen enable leads with the single pin white connectors, need to be connected together to make regen work. One of them is a ground connection, the other is the enable pin on the board.
In the software, you select the other option to the one you mention, and you also need to set the regen voltage too.
If you are running at 20 series LiPo voltages. 72 to 84 volt, then regen braking will not work with a Lyen controller unless you or he have done the regen resistor mod. Lyen does not do this as standard, you have to ask specially for it.
So with mod done, or lower battery voltages. regen jumper connected, and software set, the brake levers will give re gen as soon as they are pulled